Dehydrocorydaline Accounts the Majority of Anti-Inflammatory Property of Corydalis Rhizoma in Cultured Macrophage

Table 1

The linearity curves and content of main alkaloids in CR crude drug.

Alkaloids	Linear equation	R^2	Linear range (μg/mL)	Content (mg/g)
Columbamine	y = 43952x − 58219	0.9993	1.50 – 96.00	0.4644 ± 0.0335
Coptisine	y = 29249x − 35590	0.9994	1.45 – 93.33	0.6500 ± 0.0037
Palmatine	y = 40983x − 36074	0.9998	1.98 – 126.98	0.4337 ± 0.0117
Berberine	y = 41387x − 12535	0.9992	0.74 – 47.62	0.0952 ± 0.0067
Dehydrocorydaline	y = 37716x − 147830	0.9992	5.45 – 349.21	1.7962 ± 0.0012
Glaucine	y = 9473.5x − 9680.2	0.9990	0.69 – 44.44	1.3946 ± 0.0645
Rotundinum	y = 12804x − 5284.5	0.9999	1.5 – 96	0.5573 ± 0.0099
Corydaline	y = 10976x − 13488	0.9997	2.06 – 132.06	0.8319 ± 0.0057
Stylopine	y = 7135.6x − 12640	0.9990	0.99 – 63.56	0.6355 ± 0.0732

The linearity curves were constructed by plotting the peak area versus the concentration of each alkaloid. Each regression equation was derived from six data points (n = 6). For all alkaloids, the correlation coefficient R² > 0.999, indicating that they have good linear relationship between peak area and concentration. The main alkaloids in CR crude drug were determined (n = 3). The alkaloids, for example, corydalmine, allocryptopine, canadine, and isocorypalmine, reported to be in CR but not being detected in our HPLC analysis were not determined.