Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Tab 1

Research Article

Cytotoxic Constituents of the Bark of Hypericum roeperianum towards Multidrug-Resistant Cancer Cells

Table 1

Recorded IC50 values after 72 h incubation of drug-sensitive and MDR cancer cells lines with botanicals from the bark of Hypericum roeperianum.

Cell linesSamples, IC50 values in µg/mL, and degrees of resistance or selectivity index
HRBHRBaHRBa1HRBa2HRBa3HRBa4HRBa5HRBb

CCRF-CEM13.71±0.2615.65±1.1846.42 ± 1.9817.87±1.5316.81±4.0216.27±4.2857.15 ± 3.7648.76 ± 2.83
CEM/ADR500011.43±0.8818.56±1.74>8020.18±1.7623.03 ± 1.2213.55±0.44>8031.55 ± 2.28
Degree of resistance0.841.19>1.721.131.370.83>1.160.65
MDA-MB-231-pcDNA19.87±1.7723.12 ± 1.6572.23 ± 4.5420.42±1.7128.32 ± 1.5718.13±2.0868.91 ± 6.3048.12 ± 1.99
MDA-MB-231-BCRP18.22±0.8126.42 ± 3.0163.16 ± 3.3019.89±0.6224.41 ± 1.7717.66±1.1174.32 ± 3.9237.23 ± 4.51
Degree of resistance0.921.140.870.970.860.971.080.77
HCT116 (p53+/+)26.75 ± 1.2334.52 ± 1.7459.10 ± 3.0633.44 ± 3.6230.76 ± 1.6128.43 ± 3.01>8031.19 ± 1.26
HCT116 (p53/)17.44±2.0141.17 ± 3.14>8024.58 ± 3.4833.63 ± 3.7728.09 ± 1.75>8029.52 ± 0.98
Degree of resistance0.651.190.741.090.990.95
U87MG12.42±0.6618.03±1.7747.84 ± 4.3618.01±1.1916.13±2.0418.44±2.1147.89 ± 2.2446.55 ± 2.43
U87MG.ΔEGFR14.36±1.4820.12±0.8159.60 ± 5.1213.92±2.0718.72±1.4515.52±0.8855.20 ± 4.7928.30 ± 1.11
Degree of resistance1.161.121.250.771.160.841.150.60
HepG217.45±2.0529.17 ± 1.30>8018.75±1.4125.88 ± 1.5614.95±1.34>8069.48 ± 2.93
AML1242.09 ± 1.17>80>8061.34 ± 3.3956.12 ± 2.6765.17 ± 4.31>80>80
Selectivity index2.41>2.743.272.174.561.15

(): the degree of resistance was determined as the ratio of the IC50 value in the resistant divided by the IC50 in the sensitive cell line; CEM/ADR5000, MDA-MB-231-BCRP, HCT116 (p53/), and U87MG.ΔEGFR were used as the corresponding resistant counterpart for CCRF-CEM, MDA-MB-231-pcDNA, HCT116 (p53+/+), and U87MG, respectively; (): the selectivity index was determined as the ratio of the IC50 value in the normal AML12 hepatocytes divided by the IC50 in HepG2 hepatocarcinoma cells; in bold: significant cytotoxic effect [7, 39, 40]; nd: not determined; HRB: crude methanol extract from the bark of Hypericum roeperianum, HRBa: portion obtained by exhaustion of HRB with ethyl acetate; HRBa1-5: fractions from HRBa; HRBb: residual fraction obtained after exhaustion of HRB with ethyl acetate. The data for doxorubicin used as positive control in similar experimental conditions are shown in Table 2.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.