Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 5

Research Article

Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells

Figure 5

Carboxymethyl chitosan (CM-Ch) induced caspase-dependent apoptosis in HepG2 cells. Cells were either untreated or treated with the indicated CM-Ch (300 µg/mL) for 48 h. Lysed cells were subjected to (a) enzyme-linked immunosorbent apoptosis assay to measure histone release as an indication of apoptosis; (b) caspase-3 activity assay. Each assay was performed in triplicate, and the standard error of the mean (SEM) was calculated. Data were presented as mean ± SEM. value was calculated vs. control cells: and . Ca-CM-Ch, Chrysomya albiceps CM-Ch; Sa-CM-Ch, Sarcophaga aegyptiaca CM-Ch; sy-CM-Ch, CM-Ch of commercial chitosan.

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