Cafestol Inhibits High-Glucose-Induced Cardiac Fibrosis in Cardiac Fibroblasts and Type 1-Like Diabetic Rats

Figure 2

Cafestol modulated the Nrf2/HO-1 signaling pathway. (a) The effects of cafestol on Nrf2 and HO-1 expression were observed using western blotting. Cells were treated with 30 μM cafestol for the time indicated. Nuclear extracts were prepared, and western blotting was subsequently performed using anti-Nrf2 and anti-HO-1 antibodies. GAPDH and PARP were used as internal controls. (b) The ratio of nuclear Nrf2/cytosolic Nrf2 is presented in the bar graphs. (c) The relative protein expression of HO-1 to GAPDH is presented in the bar graphs. Data are expressed as the mean ± SEM (n = 3). compared with the control. (d) Cafestol enhanced Nrf2 nuclear translocation. Cardiac fibroblasts were grown on coverslips and treated with or without cafestol (30 µM, 12 h before undergoing high-glucose treatment for 6 h Panels are as follows: (A) control, (B) cafestol treatment, (C) high-glucose treatment, and (D) cafestol treatment and then high-glucose treatment. After treatment, cells were fixed and permeabilized, and Nrf2 subcellular localization was determined and visualized using a fluorescent microscope (taken at 200× magnification). Images representative of a typical experiment are presented.

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