Enriched-Baicalein Attenuates Allergy in Cells and Mice

Figure 4

Effect of baicalein on IL-4 (a) and IL-13 (b) production in the culture supernatant of CD3 stimulated splenocytes. Balb/c males (6 weeks old) were used and ovalbumin was injected intraperitoneally for 0, 7, 14, 21, and 28 days to Balb/c (n = 5). The baicalein, containing 100 mg of baicalein/kg body weight per day dissolved in 0.4% polyethylene glycol in 0.5% EtOH, was applied topically on a daily basis for four weeks. Cytokine measurements in the spleen cells (2 × 10⁶ mL⁻¹) were cultured for 48 hrs in a culture dish, which had been coated with CD3 mAb. Then, IL-4 and IL-13 levels were measured. The production of IL-4 and IL-13 was measured with ELISA in the culture supernatant. All measurements were made in triplicate and all values are represented as mean ± SD. Nr, normal; OVA-CT, control treated with only ovalbumin; Dexa., dexamethasone; SB, Scutellaria baicalensis Georgi extract. , , and showed a significant difference from OVA-CT.

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