Effects of Aqueous and Methanolic Extracts of Stem Bark of Alstonia boonei De Wild. (Apocynaceae) on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Wistar Rats

Table 2

IC₅₀ values of aqueous and methanolic stem-bark extracts of A. boonei on human whole blood evaluated by luminol and/or lucigenin amplified chemoluminescence, on T-cells proliferation, and on cytotoxicity on 3T3 cells.

Treatment	Oxidative burst (IC50 μg/ml)						T-cells proliferation (IC50 μg/ml)	Cytotoxicity on 3T3 cells (IC50 μg/ml)
	Luminol/zymosan			PAM/lucigenin
	WB	PMNs	MQ	WB	PMNs	MQ
Aqueous extract	10.04 ± 0.55	8.80 ± 0.24	9.48 ± 1.24	11.58 ± 0.65	8.26 ± 0.22	7.16 ± 0.13	26.46 ± 1.17	87.80 ± 1.23
Methanolic extract	7.08 ± 0,24	5.47 ± 0.03	5.98 ± 0.03	7.12 ± 0.19	6.60 ± 0.16	7.08 ± 0.27	8.19 ± 1.10	37.03 ± 0.56
Ibuprofen	15.81 ± 0.22	15.20 ± 0.64	15.69 ± 1.45	17.83 ± 0.16	15.55 ± 0.54	16.57 ± 0.54	—	—
Prednisolone	—	—	—	—	—	—	<3.10	—
Cyclohexamide	—	—	—	—	—	—	—	0.10 ± 0.13

The IC₅₀ values are presented as mean ± SD of triplicates. WB: whole blood, PMNs: polymorphonuclear neutrophils, MQ: mice peritoneal macrophages.