Evidence-Based Complementary and Alternative Medicine

Research Article

Metabolomics Analysis of Laparoscopic Surgery Combined with Wuda Granule to Promote Rapid Recovery of Patients with Colorectal Cancer Using UPLC/Q-TOF-MS/MS

Table 2

Representative serum metabolites’ RSDs of the retention time and peak area in positive and negative ion modes (n = 6).


Ion model	Metabolite	Formula	Adduct	Extraction mass (Da)	RSD% RT (min)	RSD% area

Positive	5-Hydroxytryptamine	C₁₀H₁₂N₂O	[M+H]⁺	1.06	1.06	8.49
	Tryptophan	C₁₁H₁₂N₂O₂	[M+H]⁺	0.24	0.24	3.76
	LysoPC (18:2)	C₂₆H₅₀O₇NP	[M+H]⁺	0.05	0.05	7.25
	LysoPC (16:0)	C₂₄H₅₀O₇NP	[M+H]⁺	0.06	0.06	5.98
	LysoPC (18:1)	C₂₆H₅₂O₇NP	[M+H]⁺	0.05	0.05	5.78
	LysoPC (18:0)	C₂₆H₅₄O₇NP	[M+H]⁺	0.05	0.05	3.27

Negative	Gluconic acid	C₆H₁₂O₇	[M–H]^–	195.05	0.77	10.66
	Glycocholic acid	C₂₆H₄₃NO₆	[M–H]^–	464.30	0.15	3.74
	Glycoursodeoxycholic acid	C₂₀H₃₂O₂	[M–H]^–	448.31	0.10	6.85
	LysoPE (22:6)	C₂₆H₄₃NO₅	[M–H]^–	524.28	0.07	3.14
	Arachidonic acid	C₂₀H₃₂O₂	[M–H]^–	303.23	0.03	5.93
	Palmitic acid	C₁₆H₃₂O₂	[M–H]^–	255.23	0.04	8.37

Note. RSD: relative standard deviation.