Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Table 2

ANOVA and the significance test of the response surface quadratic model for PBOM extraction.

Variables	Sum of squares	Df	Mean square	F value	value
Model	6.438	14	0.459	42.528	<0.0001^b
X1	0.061	1	0.061	5.567	0.0333^a
X2	1.111	1	1.111	102.665	<0.0001^b
X3	1.584	1	1.584	146.491	<0.0001^b
X4	0.403	1	0.403	37.298	<0.0001^b
X1, X2	2.5E − 05	1	2.5E − 05	0.003	0.9623^c
X1, X3	2.5E − 05	1	2.5E − 05	0.003	0.9623^c
X1, X4	2.5E − 05	1	2.5E − 05	0.003	0.9623^c
X2, X3	0.027	1	0.027	2.518	0.1349^c
X2, X4	0.001	1	0.001	0.021	0.8874^c
X3, X4	0.001	1	0.001	0.083	0.7772^c
	0.393	1	0.393	36.348	<0.0001^b
	1.071	1	1.071	98.954	<0.0001^b
	1.979	1	1.979	183.046	<0.0001^b
	1.357	1	1.357	125.502	<0.0001^b
Residual	0.151	14	0.011
Lack of fit	0.141	10	0.014	5.356	0.0600^c
Pure error	0.011	4	0.003
Corrected total	6.589	28

R² = 0.9769; adjusted R² = 0.9541. ^a5% significance level. ^b1% significance level. ^cNot significant relative to the pure error.