Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia by Inhibiting the Nox4/ROS Pathway

<div>Effects of BYHWD on H2O2-induced cell apoptosis in HUVECs. Cells were double-stained with cell membrane-permeable (Hoechst 33342; blue) and impermeable (PI; red) DNA labeling fluorochromes. (a) Vehicle medium (control) for 6 h. (b) Preincubation with BYHWD (30 mg/ml) for 6 h. (c) H2O2 (400 μM) for 6 h. (d) Preincubation with BYHWD (10 mg/ml) for 6 h followed by H2O2 for 6 h. (e) Preincubation with BYHWD (20 mg/ml) for 6 h followed by H2O2 for 6 h. (f) Preincubation with BYHWD (30 mg/ml) for 6 h followed by H2O2 for 6 h. (g) The percentage of apoptotic cells for each group and time point. <svg height="12.4385pt" id="M23" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -11.8015 127.857 12.4385" width="127.857pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,4.946,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g 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46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,115.008,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,121.248,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> versus the H2O2 group at the same time. Scale bars, 50 μm.</div>

Evidence-Based Complementary and Alternative Medicine

fig6

Figure 6

Figure 6: Buyang Huanwu Decoction Promotes Angiogenesis after Cerebral Ischemia by Inhibiting the Nox4/ROS Pathway 