An In Vitro Study for Evaluating Permeability and Metabolism of Kurarinone

Figure 3

Inhibition kinetic analysis of kurarinone (KU) toward recombinant UGT1A1-catalyzed 4-MU glucuronidation. (a) Concentration-dependent inhibition of UGT1A1 by kurarinone. (b) Dixon plot of kurarinone inhibition of UGT1A1-catalyzed 4-MU glucuronidation. (c) Lineweaver–Burk plot of kurarinone inhibition of UGT1A1-catalyzed 4-MU glucuronidation. (d) Secondary plot of slopes from the Lineweaver–Burk plot versus kurarinone concentrations to determine K_i. Every data point represents the mean of triplicate determinations.

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