Curative Effect and Mechanism of Guiren Runchang Granules on Morphine-Induced Slow Transit Constipation in Mice

Figure 7

(a) Result of western blot analysis. (b) Relative protein levels of SCF (% of NC). The MC group presented an obvious lower specific ratio than the NC group (31.25 ± 14.23 versus 100.00 ± 13.61). In comparison with MC group, HD and PC group showed significantly higher ratio (77.09 ± 15.77 versus 31.25 ± 14.23; 79.17 ± 14.43 versus 31.25 ± 14.23). (c) Relative protein levels of c-kit (% of NC). MC group presented strikingly lower ratio than NC group (30.44 ± 11.23 versus 100.00 ± 11.22). In comparison to MC group, the ratio of PC and HD groups was markedly higher (30.44 ± 11.23 versus 100.00 ± 11.22; 30.44 ± 11.23 versus 71.74 ± 8.32), while MD and LD groups presented no difference . (d) Relative protein levels of AQP4 (% of NC). The MC group had an obvious higher ratio than the NC group (199.22 ± 24.29 versus 99.61 ± 13.34). In contrast with the MC group, both PC and HD groups showed significant differences (199.22 ± 24.29 versus 80.08 ± 17.32; 199.22 ± 24.29 versus 103.52 ± 17.32). The MD group presented difference as well (199.22 ± 24.29 versus 152.35 ± 20.67). Quantification data was normalized to the corresponding β-actinin levels. Data are expressed as percentage of NC and mean ± SD (n = 4). versus NC group; and versus MC group.

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