Research Article
Pentagalloyl Glucose- and Ethyl Gallate-Rich Extract from Maprang Seeds Induce Apoptosis in MCF-7 Breast Cancer Cells through Mitochondria-Mediated Pathway
Figure 9
Induction of apoptosis in MCF-7 cells after MPSE treatment for 24 and 48 h (a) Determination of apoptosis in MCF-7 cells by DAPI staining. Cells were treated with MPSE at concentrations of 0, 15, 30, 60, and 120 μg/mL for 24 and 48 h. Cells were then stained by DAPI and were observed under a fluorescence microscope. (b) The percentage of apoptotic cells was analyzed by flow cytometry of Annexin-V/PI staining. (c) Column graph representing quantification of apoptotic cells. Bars representing mean ± SD values of three independent experiments. Statistically significant differences of total apoptosis from the control (). PI: propidium iodide.
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