Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress

Figure 3

The effect of AM-AS combination on HUVEC apoptosis. (a) Hoechst 33258 staining of HUVECs in CON, MOD, MOD + AM-AS (3 : 2), and MOD + SIM groups after culture for 24, 48, and 72 h. (b) Flow cytometric detection of percentage of apoptotic HUVECs in CON, MOD, MOD + AM-AS (3 : 2), and MOD + SIM groups after culture for 24 h. (c) Western blot of apoptosis-related protein caspase 3 of HUVECs in CON, MOD, MOD + AM-AS (3 : 2), and MOD + SIM groups after culture for 24 h. Data represent the mean ± SD (n = 3). vs. CON; vs. MOD. Cells in the CON group were cultured in DMEM containing 5.5 mM glucose and 10% serum from nontreated rats. Cells in the MOD, MOD + AS-AM, and MOD + SIM groups were cultured in DMEM containing 25 mM glucose plus 10% serum from nontreated, AS-AM-treated, and SIM-treated rats, respectively.

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