Effects of Huang-Lian-Jie-Du Decoction on Oxidative Stress and AMPK-SIRT1 Pathway in Alzheimer’s Disease Rat

Table 2

Primer sequences and real-time PCR conditions.

Gene	Primer sequences (5′ to 3′)	Base number	Product size	T annealing (°C)	Cycle
β-Actin	F: GAAGTGTGACGTTGACATCCG	21	282 bp	60	40
	R: GCCTAGAAGCATTTGCGGTG	20		60	40
AMPK	F: TCTCGGGGTGGTTCGGTG	18	178 bp	60	40
	R: GGGGACAGGATTTTCGGATT	20		60	40
SIRT1	F: TTCACCACAAATACTGCCAAGA	22	218 bp	60	40
	R: GATACATTACACCAAATCCTCAACA	25		60	40