Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 3


Corrigendum to “Activation of AMP-Activated Protein Kinase and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells”

Figure 3

Cells were treated with or without CB-PIC (0 or 40 μg/mL) under normoxic or hypoxic conditions for 6 h. (a) Cell lysates were prepared and subjected to western blotting to determine the expressions of AMPKα, pAMPKα, PARP, BCl-2, pERK, ERK, and β-actin. Band densities of pAMPKα, cleaved PARP, BCl-2, and pERK were quantified using Gel-pro analyzer (Media Cybernetics, Bethesda, MD, USA). Values are means ± SD, n = 3. and compared with normoxia and hypoxia groups. (b) Cells (1 × 106 cells) treated with CB-PIC for 6 hours were measured for enzyme activity of the caspase-3 class of protease in apoptotic cells by using the caspase-3 colorimetric assay kit. (c) Cells were transiently transfected with AMPKα siRNA or control siRNA in the presence or absence of CB-PIC (40 μg/mL), and PD 98059 was also treated with SW 620 in the presence or absence of CB-PIC (40 μg/mL) for 6 hours in hypoxia. Western blotting was performed to determine the expressions of PARP, AMPKα, pAMPKα, pERK, ERK, and β-actin. (d) TUNEL staining was performed and visualized under fluorescence microscopy (×200).

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