Insight into the Mechanisms Underlying the Tracheorelaxant Properties of the Sideritis raeseri Extract

Figure 3

Bradykinin potentiated, whereas L-NAME and ODQ inhibited the relaxant effects of the S. raeseri extract precontracted with CCh. Arithmetic means ± SEM (n = 6-7) of the untreated (1^st bar) and the relaxation responses precontracted with CCh (1 μM, 2^nd, 3^rd, 4^th, 5^th, and 6^th bars) without (2^nd bar) or with (3^rd, 4^th, 5^th, and 6^th bars) the S. raeseri extract (2 mg/mL) and without (3^rd bar) and with (4^th bar) additional preincubation with 100 nM bradykinin, 10 μM ODQ (5^th bar), and 100 μM L-NAME (6^th bar), respectively, for 5 min. ^∗shows statistical significance (; t-test); ^∗∗denotes significance (; t-test) between treatments as shown in the figure.