Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan-1 Pancreatic Cancer Cell Line

Figure 2

Cucurbitacin D (CuD) induces G2/M phase arrest in Capan-1 cell line: (a) CuD induces G2/M cell cycle arrest. The percentage of cell population in each phase is shown as mean ± SD from three independent experiments. , , and , significantly different as compared to the control. (b) Capan-1 and AsPC-1 cells exposed to CuD for 24 h. The expression of G2/M cell cycle arrest markers was analyzed through western blotting. The histograms indicate the relative protein expression. Results are shown as mean ± SD from three independent experiments. , , and , significantly different as compared to the control (0 µM).

(a)

(b)