Hepatoprotective Effect of Pericarpium zanthoxyli Extract Is Mediated via Antagonism of Oxidative Stress

Figure 2

Inhibition of AA + iron-induced cell death by PZE. (a) HepG2 cells were pretreated with 10–300 μg/ml PZE for 1 h and subsequently incubated with 10 μM AA for 12 h, followed by exposure to 5 μM iron for 4 h. Cell viability was measured by MTT assay. (b) Western blotting of apoptosis proteins was performed using HepG2 cell lysates incubated with 30 and 100 μg/ml PZE for 1 h, continuously treated with 10 μM AA for 12 h, and then exposed to 5 μM iron for 1 h. All data represent means ± SD of three independent experiments (, between control and AA + iron-treated cells; , between AA + iron-treated cells with or without PZE).

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