Hepatoprotective Effect of Pericarpium zanthoxyli Extract Is Mediated via Antagonism of Oxidative Stress

Figure 3

Effects of PZE on AA + iron-stimulated oxidative stress and mitochondrial dysfunction. (a) H₂O₂ production. (b) GSH contents. HepG2 cells were treated with 30 and 100 μg/ml PZE for 12 h and then continuously exposed to 10 μM AA for 12 h followed by exposure to 5 μM iron for 4 h After incubation, the GSH contents were measured in cell homogenates. (c) Mitochondrial membrane permeability was measured in HepG2 cells, pretreated with 30 and 100 μg/ml PZE for 1 h, and exposed subsequently to AA and iron. The cell population exhibiting low Rh123 staining intensity is represented as a fold. All data are presented as means ± SD of three independent experiments ( between control and AA + iron-treated cells; , between AA + iron-treated cells with or without PZE).

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