Hepatoprotective Effect of Pericarpium zanthoxyli Extract Is Mediated via Antagonism of Oxidative Stress

Figure 4

Effect of PZE on the Nrf2 nuclear translocation and the GCLC and NQO1 expressions. Western blot analysis for (a) a time (1–24 h) and (b) dose (3–100 μg/ml) response on nuclear translocation of Nrf2. HepG2 cells were incubated with 100 μg/ml PZE for 1–24 h or 3–100 μg/ml PZE for 3 h. Equal protein loadings among the samples were verified by lamin A/C immunoblotting. The relative levels of protein bands were measured by scanning densitometry. (c) Immunoblot analysis for GCLC and NQO1. HepG2 cells were treated with 100 μg/ml PZE for the indicated time period. Equal protein loadings among the samples were verified by β-actin immunoblotting. The relative levels of protein bands were measured by scanning densitometry. Data represent means ± SD of three separated experiments (, , significant as compared with control cells).

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