Gukang Capsule Promotes Fracture Healing by Activating BMP/SMAD and Wnt/β-Catenin Signaling Pathways

Table 2

PCR amplification program.

	Procedure	Annealing temp (°C)	Time	Cycle number
Stage 1	Initial denaturation	95	30 s	1
Stage 2	PCR reaction	95	3 s	40
		60	30 s
	Plate read
Stage 3	Melt curve stage