Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by <i>Alismatis rhizoma</i> Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation

<div>miRNA microarray assay shows that AD and the ERK1/2 inhibitor regulates different miRNA expressions in ox-LDL-induced VSMCs. AD and the ERK1/2 inhibitor U0126 (10 <i>μ</i>mol/L) suppressed miR-17∼92a cluster overexpression in ox-LDL-induced VSMCs. Target genes of mir-17∼92a cluster are related to TIMPs-MMPs and cell-cycle regulatory proteins according to the TargetScan7.2 database. TIMP-2, TIMP-3, and TIMP-4 are regulated by miR-20a-3p, miR-92a-1-5p, and miR-18a-3p. p21 and p27 were regulated by miR-92a-1-5p and miR-18a-3p, and the miR-17∼92a cluster acts on the p21 activated kinases (PAks) according to TargetScan7.2 database prediction.</div>

Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by <i>Alismatis rhizoma</i> Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation 

Figure 4 | Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation 