Effect of Electroacupuncture at GV20 on Sleep Deprivation-Induced Depression-Like Behavior in Mice

Figure 1

Schematic illustration of the experimental procedure. All mice were assigned into four groups: normal, S-Dep, S-Dep + EA, and S-Dep + sham EA group. A set of mice containing 10 mice were exposed to MWM training phase test during 5 consecutive days, followed by 48 h of S-Dep and EA or sham EA treatment once daily; on the 7th day after the last EA treatment, mice were used for exploring phase test. Another set of mice containing 10 mice underwent TST and FST; 3 h recovery time was given after TST. Two separated sets of mice from each group were sacrificed after S-Dep and EA/sham EA treatment for LTP/western blot or immunofluorescence.