Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans

Table 2

IC₅₀ values of cytotoxic activity against the Kato III cell line and anti-inflammatory activity by inhibiting nitric oxide production in lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells (μg/ml, n = 3).

Sample	Cytotoxic activity	Anti-inflammatory activity
	IC50 (μg/ml)	IC50 (μg/ml)	% survival
WMACE	>100	>100	113.61
EMACE	26.06 ± 1.95	82.19 ± 4.49	103.66

Cytotoxic activity against the gastric cancer cell line (Kato III) of mace extracts was assessed using the SRB assay. The amount of nitrite in the culture medium was measured by using the Griess reagent, as described in Section 2. Cytotoxicity of RAW 264.7 cell was estimated by the MTT assay.