Polygonum multiflorum Thunb. Extract Stimulates Melanogenesis by Induction of COX2 Expression through the Activation of p38 MAPK in B16F10 Mouse Melanoma Cells

Figure 4

PME treatment increased the phosphorylation of p38 MAPK and the expression of COX2 in B16F10 cells. B16F10 cells were treated with PME (2.5, 5, and 10 μg/mL) for 24 h. The protein levels in each cell lysate were estimated by western blotting. The relative expression levels of the p-p38 and COX2 were normalized to those of p38 and β-actin, respectively, and were quantified using ImageJ. Control (Con): vehicle only. Data are presented as mean ± SEM of three to four independent experiments. , vs. control.