Alleviation by Mahuang Fuzi and Shenzhuo Decoction in High Glucose-Induced Podocyte Injury by Inhibiting the Activation of Wnt/β-Catenin Signaling Pathway, Resulting in Activation of Podocyte Autophagy

Figure 2

MFSD treatment significantly reduces the damage of high glucose on podocytes. (a) Immunofluorescences for podocin and nephrin of the control group, high glucose group, DKK1 group, and MFSD group; red represents the corresponding protein and blue represents the nucleus. (b) The protein expressions of podocin, nephrin, podocalyxin, and podoplanin were analyzed by western blot. B1 represents control group, B2 represents high glucose group, B3 represents DKK1 group, and B4 represents MFSD group. (c) Quantitative statistical analysis of western blot. The obtained value of podocin, nephrin, podocalyxin, and podoplanin was normalized to the GAPDH. , , and compared with control group; , , and compared with high glucose group.

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