Antimetastatic Potential of Rhodomyrtone on Human Chondrosarcoma SW1353 Cells

Figure 6

Western blot analysis of integrin , integrin β3 protein levels, and the effector proteins. (a) Western blotting analysis of β3 integrin detected by using specific antibodies. β-Actin was used for equal loading. (b) Relative density of proteins was significantly inhibited after treatment with rhodomyrtone. (c) FAK, AKT, pAKT (Ser473), and pAKT (Thr308) were detected by using specific antibodies and β-actin is used as the loading control. (d) The relative density of proteins was significantly inhibited after treatment with rhodomyrtone in a concentration-dependent manner. (e) Specific antibodies were used for detecting Ras, RhoA, Rac1, and cdc42. β-Actin was used for equal loading. (f) The relative density of proteins was significantly inhibited after treatment with rhodomyrtone. Data are represented as mean ± standard deviation (SD) from three independent experiments. , , vs. the untreated control.

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