Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 5

Research Article

Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract

Figure 5

Effect of Agrimonia pilosa Ledeb. extract (APLE) on the levels of genes and proteins related to inflammation in lipopolysaccharide- (LPS-) induced HepG2 cells. HepG2 cells were treated with APLE (0, 100, and 200 μg/mL) in the presence and absence of LPS (100 ng/mL) for 24 h. (a) Quantitative RT-PCR for the estimation of gene expression related to inflammation (TNF-α, IL-6, and IL-1β) and an antioxidant enzyme, glutathione peroxidase (GpX). (b) Protein levels of TNF-α and GpX were assessed by immunoblot assay. The level of β-actin was shown as a loading control and used for normalization of the band intensity.
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