Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity

Figure 2

Effect of GZFLc on motor coordination and uterus inflammation. (a) GZFLc effect on motor coordination and balance of mice undergoing induced dysmenorrhea. ICR female mice were treated with estradiol for three consecutive days. The mice were randomly grouped (n = 5) and were treated with saline, ibuprofen, or GZFLc 30 min prior to being subjected to a rotarod assay. The time of a mouse staying on a rotating rod was recorded and used as a measure of GZFLc effect on induced dysmenorrhea. The experiment was performed two times independently. (b) ICR female mice were treated with estradiol for three consecutive days. On the fourth day, the animals (n = 3) were treated with ibuprofen or GZFLc 30 min prior to oxytocin injection. Twenty minutes later, the animals were killed, and the uteruses were removed and fixed in 10% formalin for histology study. Sections of 5 μ in thickness were stained with hematoxylin and eosin (H & E). The slices were examined and photographed at 4x and 10x magnifications. Con, noninduced untreated controls; Mock, oxytocin-induced and vehicle-treated; Ibup, ibuprofen at 50 mg/kg; GZFLc 150 and 300 mg/kg, respectively.

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