Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity

Figure 3

GZFLc effect on COX2 expression and COX2 activity. (a) GZFLc treatment effect on COX2 expression. Uterus tissues were removed from mock-treated or drug-treated samples (n = 2). The expression of COX2 was detected by immunoblotting analysis using β-actin as a loading control. COX2 expression was upregulated in estradiol and oxytocin-treated samples. Treatment with ibuprofen at 50 mg/kg or GZFLc at 300 mg/kg for 30 min marginally affected COX2 expression. The experiments were performed twice independently. (b) Determination of GZFLc effect on COX2 activity with an in vitro enzyme assay. The content in the GZFLc was weighed and extracted with distilled water (1.8 mg/ml) at 37°C for 2 hr. The liquid was collected by centrifugation and cleansed by filtering through a 0.2 μ filter. After serial dilution, the liquid was tested for its inhibitory activity against COX2 activity. Celecoxib was included as a positive control. Data are presented as average ± SE of duplicated samples.

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