Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 7

Research Article

Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo

Figure 7

Expression of Bcl-2, Bax, cytochrome-C, and GAPDH (loading control) detected by western blotting in spleen from healthy, control BALB/c mice (A); mice with induced leukemia (B); mice with induced leukemia treated with 100 mg/kg blank Pd-NPs (C); mice with induced leukemia treated with 50 mg/kg Pd@W.tea-NPs (D); mice with induced leukemia treated with 100 mg/kg Pd@W.tea-NPs (E); and mice with induced leukemia treated with 5 mg/kg ATRA (F). The data shown are representative of multiple blots. Abbreviations: Cyt-C, cytochrome-C; GAPHD, glyceraldehyde 3-phosphate dehydrogenase. Protein abundance was measured by densitometry using ImageJ 148-JDK 6 software for n = 3 mice per group, each measured in triplicate. and by one-way ANOVA followed by Tukey’s B-test. Values are mean ± SD relative to GAPDH (loading control).

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