Antileukemic Effect of Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract In Vitro and in WEHI-3B-Induced Leukemia In Vivo

Table 4

Bcl-2, Bax, and cytochrome-C mRNA measured and normalized by GAPDH gene by RT-qPCR in spleen from healthy (negative) control mice or mice with induced leukemia after 28 days of treatment as stated.

mRNA	Control	Leukemia	Blank NPs (100 mg/kg)	[email protected] (50 mg/kg)	[email protected] (100 mg/kg)	ATRA (5 mg/kg)
Bcl-2	12.5 ± 0.11	33.01 ± 1.15	31.23 ± 0.75	25.25 ± 0.25	21.12 ± 0.44	16.98 ± 1.1
Bax	14.07 ± 1.7	11.34 ± 1.0	11.55 ± 0.35	25.24 ± 0.20	31.10 ± 0.17	42.10 ± 2.2
Cyt-C	12.0 ± 1.15	8.60 ± 0.75	9.25 ± 0.25	21.54 ± 0.30	26.45 ± 0.05	34.05 ± 2.1

Values are mean ± SD relative to GAPDH (loading control) measured by densitometry using ImageJ 148-JDK 6 software for n = 3 mice per group, each measured in triplicate. and by one-way ANOVA followed by Tukey’s B-test.