Evaluation of Immunomodulatory and Antiarthritic Potential of Trigonella gharuensis Extracts

Table 1

Primer sequences.

Genes	Forward primer	Reverse primer	Product size	Reference
IL-4	5′-CACCTTGCTGTCACCCTGTT-3′	5′-TCACCGAGAACCCCAGACTT-3′	231	[18]
IL-6	5′-CCCACCAAGAACGATAGTCA-3′	5′-CTCCGACTTGTGAAGTGGTA-3′	247	[26]
TNF-α	5′-AGTCCGGGCAGGTCTACTTT-3′	5′-GGAAATTCTGAGCCCGGAGT-3′	202	NM_012675.3
NF-κB	5′-TGAGATCCATGCCATTGGCC-3′	5′-AGCTGAGCATGAAGGTGGATG-3′	207	[18]
COX2	5′-CCAGATGGCCAGAGGACTCA-3′	5′-TGTGAGTCCCGAGGGAATAGA-3′	237	[18]
IL-1β	5′-GCTGTCCAGATGAGAGCATC-3′	5′-GTCAGACAGCACGAGGCATT-3′	293	[19]
GAPDH	5′-GTCATCAACGGGAAACCCAT-3′	5′-ATCACAAACATGGGGGCATC-3′	197	NM_017008.4