Ginsenoside Rg1 Drives Stimulations of Timosaponin AIII-Induced Anticancer Effects in Human Osteosarcoma Cells

Figure 4

Synergistic effects of Rg1 on TA3-induced antimetastatic characteristics of MG63 human osteosarcoma cells. (a) Rg1 and TA3 synergistically attenuate migration of MG63 cells. Recovery of the wound width on the center of the cell surface was inhibited most when the cells were treated with Rg1 and TA3 together. Cells were treated with 0.1% FBS for negative control. The wound widths were measured for the quantitation. (b) Rg1 and TA3 synergistically inhibit gelatinase activities of both gelatinases (MMP-2 and MMP-9). (c) Transcriptional expressions of both enzymes (MMP-2 and MMP-9) were downregulated further by the combinatorial treatment of Rg1 (250 μM) and TA3 (6 μM) in MG63 cells. The results were analyzed statistically by ANOVA and Tukey’s test (, , vs control, vs treatment of TA3, vs treatment of Rg1).

(a)

(b)

(c)