Ginsenoside Rg1 Drives Stimulations of Timosaponin AIII-Induced Anticancer Effects in Human Osteosarcoma Cells

Figure 6

Stimulations of Rg1 on TA3-induced cytotoxic effect and apoptosis in U2OS human osteosarcoma cells. (a) Cytotoxic effect of Rg1 plus TA3 was monitored in U2OS cells. The cells were treated with each chemical compound for 24 h: TA3 (6 μM), Rg1 (250 μM), TA3 (6 μM) plus Rg1 (250 μM) for 24 h. Then, the samples were subjected to the cell viability assay using the CCK-8 kit. (b) Apoptosis of U2OS cells was synergistically induced by Rg1 and TA3. TA3 (6 μM) and/or Rg1 (250 μM) were applied to the cells for 24 h. Then, Annexin V/PI apoptosis assay was performed. (c) The results from Annexin V/PI apoptosis assay were quantified. (d) DNA fragmentation was synergistically induced by Rg1 and TA3. TA3 (6 μM) and/or Rg1 (250 μM) were applied to the cells for 24 h. Then, the TUNEL assay was performed. The results were statistically evaluated using ANOVA and Tukey’s test (, vs control, vs treatment of TA3, vs treatment of Rg1).

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