Ginsenoside Rg1 Drives Stimulations of Timosaponin AIII-Induced Anticancer Effects in Human Osteosarcoma Cells

<div>Stimulations of Rg1 on TA3-induced antimetastatic effects in U2OS human osteosarcoma cells. (a) Rg1 and TA3 synergistically attenuate the migration of U2OS cells. Recovery of the wound width on the center of the cell surface was inhibited most when the cells were treated with Rg1 and TA3 together. Cells were treated with 0.1% FBS for negative control. The wound widths were measured for the quantitation. (b) Rg1 and TA3 synergistically inhibit gelatinase activities of MMP-2 and MMP-9. (c) Transcriptional expressions of MMP-2 and MMP-9 were downregulated further by the combinatorial treatment of Rg1 (250 <i>μ</i>M) and TA3 (6 <i>μ</i>M) in U2OS cells. 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-12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.785,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,50.025,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs control, <svg height="12.8176pt" id="M16" style="vertical-align:-0.6371002pt" version="1.1" viewbox="-0.0498162 -12.1805 49.7218 12.8176" width="49.7218pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M263 363V584C343 572 363 521 380 464L408 470C399 526 392 562 387 596C376 598 367 601 353 604C328 612 296 617 263 617V693H228V615C122 603 67 532 67 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287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs treatment of Rg1).</div>

Evidence-Based Complementary and Alternative Medicine

fig7

Figure 7

Figure 7: Ginsenoside Rg1 Drives Stimulations of Timosaponin AIII-Induced Anticancer Effects in Human Osteosarcoma Cells 