Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 2

Research Article

Zi Shen Huo Luo Formula Prevents Aldosterone-Induced Cardiomyocyte Hypertrophy and Cardiac Fibroblast Proliferation by Regulating the Striatin-Mediated MR/EGFR/ERK Signaling Pathway

Figure 2

Effects of aldosterone induction time on the cardiomyocyte surface area and proliferation and hydroxyproline secretion of cardiac fibroblasts. Cardiomyocytes were treated with 10−9 mol/l aldosterone, and cardiac fibroblasts were treated with 10−7 mol/l aldosterone for 0, 5, 15, 30, 60 or 120 min. (a) Cells were immunofluorescence stained with DAPI (blue), and cardiomyocytes were detected by immunofluorescence staining using FITC-conjugated α-actinin (green). (b) Cardiomyocyte surface area (relative to that in the untreated group) was determined by α-actinin immunofluorescence staining. (c) The OD values of cardiac fibroblasts were detected by a CCK-8 assay. (d) Hydroxyproline content in the cardiac fibroblast culture supernatant. vs. the untreated group; vs. the group treated for 5 min; vs. the group treated for 15 min; vs. the group treated for 30 min; and vs. the group treated for 1 h.
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