Zi Shen Huo Luo Formula Prevents Aldosterone-Induced Cardiomyocyte Hypertrophy and Cardiac Fibroblast Proliferation by Regulating the Striatin-Mediated MR/EGFR/ERK Signaling Pathway

Figure 4

Effect of ZSHLF on striatin mRNA expression and interaction between the striatin and MR proteins in cardiomyocytes and cardiac fibroblasts. In the blank group, the cells were incubated with blank serum-containing medium. In the ALD group, cardiomyocytes and cardiac fibroblasts were pretreated with blank serum, the cardiomyocytes were then incubated with 10⁻⁹ mol/l aldosterone for 30 min, and the cardiac fibroblasts were incubated with 10⁻⁷ mol/l aldosterone for 30 min. In the ZSHLF group, cardiomyocytes and cardiac fibroblasts were pretreated with 10% ZSHLF-containing serum for 2 h and then incubated with aldosterone as described for the ALD group. (a) mRNA expression levels of striatin in cardiomyocytes and cardiac fibroblasts were detected by qRT-PCR. The 2^−ΔΔct method was used for relative quantitative analysis of mRNA expression levels. (b) Protein bands containing striatin and MR from cardiomyocytes were detected by IP. (c) Protein bands containing striatin and MR from the cardiac fibroblasts were detected by IP. (d) Protein expression of MR and striatin in the cardiomyocytes. (e) Protein expression of MR and striatin in cardiac fibroblasts. vs. the blank group. vs. the ALD group.

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