[Retracted] Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction

Figure 4

Effects of CQCQD and CCh on mRNA and protein expression of IL-1β, TNF-α, and CPI-17 in jejunum smooth muscle strips. Rats received 2 intraperitoneal injections of L-ornithine (Orn; 3.0 g/kg; pH 7.0) at 1 hour apart and controls received the same regimen of normal saline injections. In the treatment groups, rats either received single intraperitoneal injection of carbachol (CCh; 60 μg/kg) or 3 times oral gavage of chaiqin chengqi decoction (CQCQD; 20 g/kg) at 2-hourly interval begun at 24 hours after the first L-ornithine injection. Rats were sacrificed at 24 or 30 hours to isolate jejunum smooth muscle strips. (a) Representative images of agarose gel electrophoresis of targeted RT-PCR mRNAs. (b) Histograms for semiquantitative mRNA expression. (c) Representative western-blotting images of proteins. (d) Histograms for semiquantitative protein expression. (e) Example images of immunofluorescence (s, smooth muscle layer; m, mucosa; sm, submucosa). Relative expression of individual mRNA and protein are calculated according to β-actin. The bar of medium and sparse patterns represents rats sacrificed at 24 and 30 hours after the first L-ornithine/saline injection, respectively. vs. saline group, vs. L-ornithine group; vs. CCh-treated group. Values are means ± SEM of 4 animals per group.

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