Ulmus parvifolia Jacq. Exhibits Antiobesity Properties and Potentially Induces Browning of White Adipose Tissue

Figure 5

UP increased expression of browning markers in mice. Extracted RNA was reverse transcribed, and the browning related genes UCP-1 and PGC-1α were investigated using Real-Time PCR conducted using the resultant cDNA (a). Quantification of gel images (c and e). Protein expressions of p-AMPKα and p-ACC in liver tissue were investigated using western blot analysis, as shown in (b), and the gel images were quantified using ImageJ (d and f). All experiments were conducted in triplicate. Statistics were analyzed using one-way ANOVA, and was considered significant.

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