Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α

Figure 2

Effect of GBEE on HL-60 cell adhesion to EA.hy926 cells and analysis of TNF-α-induced changes in EA.hy926 cell height. EA.hy926 cells (5 × 10⁴ cells/30 mm plate) were seeded, cultured overnight, and treated with 10, 50, or 100 μg/mL GBEE for 8 h followed by stimulation with or without 10 ng/mL TNF-α for 3 h. (a) HL-60 cells were prestained with 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, and the adhesion of HL-60 cells to EA.hy926 cells was observed under a fluorescence microscope. (b) EA.hy926 cell staining was observed using a fluorescence reader with an excitation wavelength of 485 nm and an emission wavelength of 530 nm. (c) EA.hy926 cell height was observed using AFM. The height of the control cells was set at 100%. Values were presented as means ± SDs (n = 3). ^abcValues not sharing the same letter were significantly different, as shown by Duncan’s test ().

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