Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α

Figure 3

Expression levels of TNF-α-induced adhesion molecules and cell junction proteins. EA.hy926 cells (5 × 10⁴ cells/30 mm plate) were seeded, cultured overnight, and treated with 10, 50, or 100 μg/mL GBEE for 8 h followed by stimulation with or without 10 ng/mL TNF-α for 3 h. (a) Immunoblot assays were performed to determine the expression levels of ICAM-1, PECAM-1, p-VE-cadherin, and VE-cadherin in EA.hy926 cells. (b) Protein expression in EA.hy926 cells was quantified using densitometry. The expression levels in the control group were set at 100%. (c) The effect of GBEE on transepithelial electrical resistance in EA.hy926 cells. Values were presented as means ± SDs (n = 3). ^abcValues not sharing the same letter were significantly different, as shown by Duncan’s test ().

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