Suppressive Effects of the Gynura bicolor Ether Extract on Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cells Induced by TNF-α

Figure 4

Effect of GBEE on transmigration regulatory molecules in EA.hy926 cells. EA.hy926 cells (5 × 10⁴ cells/30 mm plate) were seeded, cultured overnight, and treated with 100 μg/mL GBEE for 8 h followed by stimulation with or without 10 ng/mL TNF-α for 3 h. (a) The effect of GBEE on ROS levels in EA.hy926 cells was determined. (b) The expression levels of p-Src, Src, RhoA, p-ERM, ROCK, and ERM in EA.hy926 cells were determined using the immunoblot assay. (c) The protein expression in EA.hy926 cells was quantified using densitometry; expression levels in the control group were set at 100%. Values were presented as means ± SDs (n = 3). ^abcValues not sharing the same letter were significantly different, as shown by Duncan’s test ().

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