Natural Compound Mixture, Containing Emodin, Genipin, Chlorogenic Acid, Cimigenoside, and Ginsenoside Rb1, Ameliorates Psoriasis-Like Skin Lesions by Suppressing Inflammation and Proliferation in Keratinocytes

Figure 3

Effects of PSM on the proinflammatory chemokine expression and related signaling pathways in M5-stimulated HaCaT cells. (a) HaCaT cells were pretreated with emodin, genipin, chlorogenic, cimigenoside, ginsenoside Rb1 (10 μM), PSM (50 μM), or dexamethasone (DEX, 10 μM) for 1 h and then stimulated with M5 (IL-1α, IL-17A, IL-22, oncostatin M, and TNF-α, 10 ng/ml each) for 24 h. The CXCL8, CXCL10, and CCL20 levels in the culture supernatants were determined using commercial ELISA kits. (b) The effects of PSM on cell viability was assessed using an XTT assay. HaCaT cells were treated with emodin, genipin, chlorogenic, cimigenoside, ginsenoside Rb1, or PSM (10, 50 μM) for 24 h. (c) HaCaT cells were pretreated with PSM (50 μM) or DEX (10 μM) for 1 h and then stimulated with M5 for 30 min. Protein expression of p-ERK, p-p38, p-JNK, and p-STAT3 were assessed by Western blotting, and the band intensities were normalized versus ERK, p38, JNK, and STAT3, respectively. (d) HaCaT cells were pretreated with U0126, SB202190, SP600125, and cryptotanshinone (10 μM) for 1 h and then stimulated with M5 for 24 h. The CXCL8, CXCL10, and CCL20 levels in the culture supernatants were determined using commercial ELISA kits. vs. normal controls. vs. M5-treated cells. vs. M5 + PSM-treated cells.

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