In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice

Table 4

Activity of topical application of the solvent fraction extract ointment of the flower of Hagenia abyssinica on wound contraction of excision wound model in mice.

Wound area (mm^2) (mean ± SEM) (% contraction)
Day	SO	NF	5% AQFO	10% AQFO	5% CHFO	10% CHFO	5% EAFO	10% EAFO
0	312.67 ± 1.09	311.33 ± 0.71	312.25 ± 0.95	312.67 ± 0.76	312.67 ± 0.56	311.83 ± 0.87	312.83 ± 0.79	312.00 ± 0.77
2	304.17 ± 3.49 (2.72%)	288.54 ± 2.96 (7.29%)^a1	296.88 ± 2.68 (4.92%)	288.54 ± 2.98 (8.72%)^a1	300 ± 2.28 (4.05%)	296.88 ± 3.52 (4.79)	293.75 ± 3.23 (6.10%)	286.46 ± 2.98 (8.19%)^a2
4	286.46 ± 3.49 (8.38%)	251.04 ± 2.98 (19.37%)^a2	271.86 ± 2.77 (12.94%)^a1d3	259.36 ± 3.52 (17.05%)^a2	276.04 ± 2.51 (11.72%)^b3c2d3e1	270.83 ± 3.09 (13.15)^a1b2d3	261.46 ± 2.98 (16.42%)^a2d1	247.92 ± 3.09 (20.54%)^a3
6	201.04 ± 2.98 (35.70%)	173.96 ± 3.78 (44.12%)^a3	184.38 ± 3.52 (40.95%)^a1d3	170.83 ± 2.08 (45.36%)^a3	189.58 ± 4.17 (39.37%)^b1c2d3	185.42 ± 2.64 (40.54)^a1c1d3	186.46 ± 2.98 (40.40%)^a1c1d3	162.50 ± 2.28 (47.92%)^a3
8	98.96 ± 1.92 (68.35%)	75.00 ± 3.76 (75.91%)^a3	81.25 ± 2.28 (73.98%)^a2c1d3	67.71 ± 2.98 (78.34%)^a3	81.25 ± 2.28 (74.01%)^a1c1d3	80.21 ± 2.98 (74.28)^a1d3	78.13 ± 2.68 (75.02%)^a2d3	55.21 ± 2.98 (82.30%)^a3b3
10	71.88 ± 2.68 (77.01%)	35.42 ± 3.09 (88.62%)^a3	51.04 ± 2.28 (83.65%)^a2b1c2d3	31.25 ± 2.28 (90.01%)^a3	55.21 ± 1.92 (82.34%)^a1b2c3d3e1	39.58 ± 2.08 (87.31)^a3d2	41.69 ± 4.47 (86.67%)^a3d3	19.79 ± 3.76 (93.66%)^a3b1
12	43.75 ± 2.68 (86.01%)	10.42 ± 3.49 (96.65%)^a3	26.04 ± 2.98 (91.66%)^a2b2d2	16.67 ± 2.64 (94.67%)^a3	30.21 ± 3.76 (90.34%)^a1b3c1d3	22.92 ± 2.08 (92.65)^a3d2	20.33 ± 1.94 (93.50%)^a3d1	9.37 ± 2.68 (97.66%)^a3
14	25.00 ± 2.28 (92.00%)	3.13 ± 2.13 (100%)	10.38 ± 3.48 (96.68%)^a2	2.08 ± 1.32 (99.33%)^a3	13.54 ± 2.98 (95.67%)^a1b2c1d2	9.38 ± 3.13 (97.00)^a2	7.33 ± 1.92 (97.66%)^a3	1.04 ± 1.04 (99.67%)^a3
16	13.54 ± 2.98 (95.67%)	0 (100%)	3.13 ± 2.13 (99.00%)^a2	0 (100%)^a3	3.13 ± 2.13 (99.00%)^a2	1.08 ± 1.04 (99.65)^a3	0 (100%)^a3	0 (100%)^a3

Note. Values are expressed as mean ± SEM (n = 6 mice in each group) and analyzed by one-way ANOVA followed by the post hoc Tukey test; ^acompared to the negative control; ^bcompared to positive control; ^ccompared to 10% AQFO; ^dcompared to 10% EAFO; ^ecompared to 5% EAFO; ¹; ²; ³; AQFO = aqueous fraction ointment; CHFO = chloroform fraction ointment; EAFO = ethylacetate fraction ointment; NF = nitrofurazone 0.2% ointment; SO = simple ointment.