In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice
Table 4
Activity of topical application of the solvent fraction extract ointment of the flower of Hagenia abyssinica on wound contraction of excision wound model in mice.
Wound area (mm2) (mean ± SEM) (% contraction)
Day
SO
NF
5% AQFO
10% AQFO
5% CHFO
10% CHFO
5% EAFO
10% EAFO
0
312.67 ± 1.09
311.33 ± 0.71
312.25 ± 0.95
312.67 ± 0.76
312.67 ± 0.56
311.83 ± 0.87
312.83 ± 0.79
312.00 ± 0.77
2
304.17 ± 3.49 (2.72%)
288.54 ± 2.96 (7.29%)a1
296.88 ± 2.68 (4.92%)
288.54 ± 2.98 (8.72%)a1
300 ± 2.28 (4.05%)
296.88 ± 3.52 (4.79)
293.75 ± 3.23 (6.10%)
286.46 ± 2.98 (8.19%)a2
4
286.46 ± 3.49 (8.38%)
251.04 ± 2.98 (19.37%)a2
271.86 ± 2.77 (12.94%)a1d3
259.36 ± 3.52 (17.05%)a2
276.04 ± 2.51 (11.72%)b3c2d3e1
270.83 ± 3.09 (13.15)a1b2d3
261.46 ± 2.98 (16.42%)a2d1
247.92 ± 3.09 (20.54%)a3
6
201.04 ± 2.98 (35.70%)
173.96 ± 3.78 (44.12%)a3
184.38 ± 3.52 (40.95%)a1d3
170.83 ± 2.08 (45.36%)a3
189.58 ± 4.17 (39.37%)b1c2d3
185.42 ± 2.64 (40.54)a1c1d3
186.46 ± 2.98 (40.40%)a1c1d3
162.50 ± 2.28 (47.92%)a3
8
98.96 ± 1.92 (68.35%)
75.00 ± 3.76 (75.91%)a3
81.25 ± 2.28 (73.98%)a2c1d3
67.71 ± 2.98 (78.34%)a3
81.25 ± 2.28 (74.01%)a1c1d3
80.21 ± 2.98 (74.28)a1d3
78.13 ± 2.68 (75.02%)a2d3
55.21 ± 2.98 (82.30%)a3b3
10
71.88 ± 2.68 (77.01%)
35.42 ± 3.09 (88.62%)a3
51.04 ± 2.28 (83.65%)a2b1c2d3
31.25 ± 2.28 (90.01%)a3
55.21 ± 1.92 (82.34%)a1b2c3d3e1
39.58 ± 2.08 (87.31)a3d2
41.69 ± 4.47 (86.67%)a3d3
19.79 ± 3.76 (93.66%)a3b1
12
43.75 ± 2.68 (86.01%)
10.42 ± 3.49 (96.65%)a3
26.04 ± 2.98 (91.66%)a2b2d2
16.67 ± 2.64 (94.67%)a3
30.21 ± 3.76 (90.34%)a1b3c1d3
22.92 ± 2.08 (92.65)a3d2
20.33 ± 1.94 (93.50%)a3d1
9.37 ± 2.68 (97.66%)a3
14
25.00 ± 2.28 (92.00%)
3.13 ± 2.13 (100%)
10.38 ± 3.48 (96.68%)a2
2.08 ± 1.32 (99.33%)a3
13.54 ± 2.98 (95.67%)a1b2c1d2
9.38 ± 3.13 (97.00)a2
7.33 ± 1.92 (97.66%)a3
1.04 ± 1.04 (99.67%)a3
16
13.54 ± 2.98 (95.67%)
0 (100%)
3.13 ± 2.13 (99.00%)a2
0 (100%)a3
3.13 ± 2.13 (99.00%)a2
1.08 ± 1.04 (99.65)a3
0 (100%)a3
0 (100%)a3
Note. Values are expressed as mean ± SEM (n = 6 mice in each group) and analyzed by one-way ANOVA followed by the post hoc Tukey test; acompared to the negative control; bcompared to positive control; ccompared to 10% AQFO; dcompared to 10% EAFO; ecompared to 5% EAFO; 1;2;3; AQFO = aqueous fraction ointment; CHFO = chloroform fraction ointment; EAFO = ethylacetate fraction ointment; NF = nitrofurazone 0.2% ointment; SO = simple ointment.