Oridonin Attenuates Lipopolysaccharide-Induced ROS Accumulation and Inflammation in HK-2 Cells

Figure 3

Oridonin attenuated LPS-induced NF-κB nuclear translocation. HK-2 cells cultured and treated as above were collected, and cytosolic and nuclear fractions were isolated as described in Materials and Methods. Western blot analysis was performed to detect the subcellular localization of NF-κB using an antibody against the NF-κB subunit p65. β-actin was used as a cytosolic marker and fibrillarin served as a nuclear marker.