In Vitro and In Vivo Anti-Inflammatory Activities of Benjakul: A Potential Medicinal Product from Thai Traditional Medicine

Table 3

Marker contents and anti-inflammatory activity of BJK extract after the stability test.

Testing	Marker content mean (mg/g) ± SD						IC50 on PGE2 inhibition^d(mean µg/mL ± SEM)
	Plumbagin		Piperine^b		Myristicin^c
	Content^a(mg/g)	% remaining	Content^b(mg/g)	% remaining	Content^c(mg/g)	% remaining
BJK day 0	0.86 ± 0.03	100	104.83 ± 10.11	100	4.03 ± 0.09	100	4.42 ± 0.34
BJK day 15	0.83 ± 0.01	97.35	94.20 ± 2.48	89.86	2.99 ± 0.05	74.25	5.24 ± 0.23
BJK day 30	0.81 ± 0.01	94.81	105.88 ± 0.40	101.00	3.54 ± 0.11	87.92	4.64 ± 0.18
BJK day 60	ND	ND	84.52 ± 1.36	80.62	3.10 ± 0.04	76.85	4.23 ± 1.10
BJK day 90	ND	ND	93.12 ± 0.76	88.83	3.02 ± 0.02	74.91	4.18 ± 0.19
BJK day 120	ND	ND	95.18 ± 0.65	90.79	3.28 ± 0.06	81.33	4.47 ± 0.72
BJK day 180	ND	ND	88.98 ± 1.65	84.88	3.69 ± 0.03	91.53	1.76 ± 0.15

; each sample was compared with BJK day 0 using One-way ANOVA statistical analysis. ^aData were calculated following the standard linear equation: y = 52.702x–333.81, R² = 0.9982. ^bData were calculated following the standard linear equation: y = 22.452x–1500.8, R² = 0.9997. ^cData were calculated following the standard linear equation: y = 7.0348x–117.11, R² = 0.9991.