Maren Pills Improve Constipation via Regulating AQP3 and NF-κB Signaling Pathway in Slow Transit Constipation In Vitro and In Vivo

Figure 7

Effect of Maren pills on the expression of AQP3, CFTR, and PKA proteins in HT-29 cells. HT-29 cells were incubated with Maren pills or combined with PMA for 24 h. (a) The expressions of AQP3, CFTR, and PKA proteins in HT-29 cells were detected by western blot. (b) The localization of AQP3 (green) in HT-29 cells was determined by immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar, 20 µm). The results were presented as the mean ± standard deviation. and , compared with the control II group. , compared with the Maren pills group.

(a)

(b)