Evidence-Based Complementary and Alternative Medicine

Research Article

Evaluation of Subchronic Toxicity and Genotoxicity of Ethanolic Extract of Aster glehni Leaves and Stems

Table 6

Results of bacterial reverse mutation assay.


Test article	Dose (μg/plate)	Colonies/plate [factor]^a
		TA98		TA100		TA1535		TA1537		WP2 uvrA
		−S9 mix	+S9 mix	−S9 mix	+S9 mix	−S9 mix	+S9 mix	−S9 mix	+S9 mix	−S9 mix	+S9 mix

EAG	0	26 ± 4	25 ± 3	114 ± 8	107 ± 9	14 ± 2	15 ± 2	15 ± 2	15 ± 2	22 ± 3	29 ± 4
	0	—	—	—	—	—	—	—	—	—	—
	50	25 ± 6	28 ± 3	111 ± 6	102 ± 9	12 ± 4	16 ± 5	13 ± 2	13 ± 3	22 ± 4	25 ± 4
	50	[1.0]	[1.1]	[1.0]	[0.9]	[0.9]	[1.1]	[0.9]	[0.9]	[1.0]	[0.9]
	150	27 ± 4	30 ± 5	107 ± 15	120 ± 16	10 ± 1	15 ± 1	15 ± 1	12 ± 3	18 ± 3	26 ± 7
	150	[1.1]	[1.2]	[0.9]	[1.1]	[0.8]	[1.0]	[1.0]	[0.8]	[0.8]	[0.9]
	500	34 ± 5	34 ± 4	103 ± 7	105 ± 5	13 ± 1	17 ± 1	13 ± 1	14 ± 1	20 ± 2	26 ± 6
	500	[1.3]	[1.4]	[0.9]	[1.0]	[1.0]	[1.1]	[0.9]	[1.0]	[0.9]	[0.9]
	1,500	26 ± 5	40 ± 3	109 ± 6	120 ± 10	11 ± 1	15 ± 2	12 ± 2	13 ± 2	23 ± 4	26 ± 4
	1,500	[1.0]	[1.6]	[1.0]	[1.1]	[0.8]	[1.0]	[0.8]	[0.9]	[1.0]	[0.9]
	3,000	34 ± 5	56 ± 5	121 ± 2	137 ± 1	13 ± 2	13 ± 3	13 ± 3	17 ± 3	22 ± 2	27 ± 2
	3,000	[1.3]	[2.3]	[1.1]	[1.3]	[0.9]	[0.9]	[0.9]	[1.2]	[1.0]	[0.9]
	5,000	34 ± 2	63 ± 6	116 ± 12	137 ± 3	13 ± 1	13 ± 1	12 ± 2	20 ± 1	25 ± 2	23 ± 3
	5,000	[1.3]	[2.5]	[1.0]	[1.3]	[0.9]	[0.8]	[0.8]	[1.3]	[1.1]	[0.8]
Positive control^b		225 ± 16	118 ± 8	465 ± 60	1504 ± 102	408 ± 12	142 ± 19	265 ± 20	182 ± 22	227 ± 25	104 ± 8
Positive control^b		[8.8]	[4.8]	[4.1]	[14.0]	[29.9]	[9.3]	[18.1]	[12.1]	[10.3]	[3.6]

Data are expressed as mean ± standard deviation. ^aThree plates were used each dose. Factor = no. of colonies of treated plate/no. of colonies of negative control plate. ^bTA98: 2-NF 2 μg/plate (−S9 mix), B[a]P 1 μg/plate (+S9 mix); TA100:SA 0.5 μg/plate (−S9 mix), 2-AA 1 μg/plate (+S9 mix); TA1535:SA 0.5 μg/plate (−S9 mix), 2-AA 2 μg/plate (+S9 mix); TA1537:ICR-191 0.5 μg/plate (−S9 mix), 2-AA 1 μg/plate (+S9 mix); and WP2 uvrA:4NQO 0.5 μg/plate (−S9 mix), 2-AA 6 μg/plate (+S9 mix). 2-NF, 2-nitrofluorene; B[a]P, benzo[a]pyrene; SA, sodium azide; 2-AA, 2-aminoanthracene; ICR-191, acridine mutagen ICR 191; and 4NQO, 4-nitroquinoline N-oxide.